prokka
https://github.com/tseemann/prokka
Perl
:zap: :aquarius: Rapid prokaryotic genome annotation
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- Issues
- Unusually high ncRNA detected on same contig & coordinate - Archea
- prokka setup db
- Bio::SearchIO: hmmer3 cannot be found
- Cannot find annotations for viral genomes
- Can not run genus specific database
- gbff files
- perl: symbol lookup error Perl_xs_apiversion_bootcheck
- bioperl v1.7.8 kills the prokka πΏπ«
- gene in faa and ffn starts with different amino acid.
- rna and CDS overlapped with no --cdsrnaolap set
- Docs
- Perl not yet supported